Dexrazoxane may prevent doxorubicin-induced DNA damage via depleting both Topoisomerase II isoforms

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Topoisomerase IIbeta mediated DNA double-strand breaks: implications in doxorubicin cardiotoxicity and prevention by dexrazoxane.

Doxorubicin is among the most effective and widely used anticancer drugs in the clinic. However, cardiotoxicity is one of the life-threatening side effects of doxorubicin-based therapy. Dexrazoxane (Zinecard, also known as ICRF-187) has been used in the clinic as a cardioprotectant against doxorubicin cardiotoxicity. The molecular basis for doxorubicin cardiotoxicity and the cardioprotective ef...

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Dexrazoxane exacerbates doxorubicin-induced testicular toxicity.

Infertility induced by anti-cancer treatments pose a major concern for cancer survivors. Doxorubicin (DXR) has been previously shown to exert toxic effects on the testicular germinal epithelium. Based upon the cardioprotective traits of dexrazoxane (DEX), we studied its potential effect in reducing DXR-induced testicular toxicity. Male mice were injected with 5  mg/kg DXR, 100  mg/kg DEX, combi...

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Both and Isoforms of Mammalian DNA Topoisomerase II Associate with Chromosomes in Mitosis

Two isoforms of DNA topoisomerase II, and , coded by separate genes, are expressed in actively cycling vertebrate cells. Some previous studies have suggested that only topoisomerase II remains associated with chromosomes at mitosis. Here, the distributions of topoisomerase II and in mitosis were studied by subcellular fractionation and by immunolocalization. Both isoforms of topoisomerase II we...

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Ultraviolet-induced DNA Damage Stimulates Topoisomerase

An antibody-based method was used to examine genomic DNA cleavage by endogenous topoisomerases in living cells. The method quantifies cleavable (covalent) complex formation in vivo after exposure to topoisomerase poisons, as reported previously (D. Subramanian et al., Cancer Res., 55: 2097-2103, 1995). Unexpectedly, exposing cells to UVB irradiation stim ulated endogenous topoisomerase I-DNA co...

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Dexrazoxane does not protect against doxorubicin-induced damage in young rats.

Doxorubicin (DOX), an anticancer drug, causes a dose-dependent cardiotoxicity. Some evidence suggests that female children have an increased risk for DOX-mediated cardiac damage. To determine whether the iron chelator dexrazoxane (DXR) could reduce DOX-induced cardiotoxicity in the young, we injected day 10 neonate female and male rat pups with a single dose of saline or DOX, DXR, or DXR + DOX ...

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ژورنال

عنوان ژورنال: BMC Cancer

سال: 2014

ISSN: 1471-2407

DOI: 10.1186/1471-2407-14-842